In February 2016 investigators at the Thomas Jefferson Health District (Virginia) investigated an outbreak of Clostridium perfringens among individuals who attended a special "Ramen Night" at a local restaurant. Among 30 restaurant patrons with diarrheal illness, 24 (80%) reported consuming tonkotsu ramen. Clinical testing of the stools revealed elevated levels of Clostridium spp. C. Perfringens isolated from four stool samples and the leftover tonkotsu ramen composite were indistinguishable by PFGE. Environmental Health Specialists determined that the ramen had not been properly cooled after preparation and observed limited accessibility to a hand sink as well as improper hand washing technique.