An outbreak of non-O157 Shiga toxin–producing Escherichia coli occurred among students at a high school in Minnesota, in November 2010. The students had handled and consumed venison from a wild white-tailed deer in a high school class. Consuming undercooked venison and not washing hands after handling raw venison were associated with illness. E. coli O103:H2 (2 isolates) and non-Shiga toxin–producing E. coli O145:NM(1 isolate) were isolated from ill students and the venison.